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KMID : 0352720160400020185
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Journal of Ginseng Research
2016 Volume.40 No. 2 p.185 ~ p.195
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Pectinase-treated Panax ginseng ameliorates hydrogen peroxide-induced oxidative stress in GC-2 sperm cells and modulates testicular gene expression in aged rats
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Spandana Rajendra Kopalli
Cha Kyu-Min
Jeong Min-Sik
Lee Sang-Ho
Sung Jong-Hwan
Seo Seok-Kyo
Kim Si-Kwan
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Abstract
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Background: To investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male
subfertility animal models.
Methods: Hydrogen peroxide (H2O2)-induced mouse spermatocyte GC-2spd cells were used as an in vitro
model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with
a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein
expression level of antioxidative and spermatogenic genes in young and aged control rats were
compared using real-time reverse transcription polymerase chain reaction and western blotting.
Results: GINST treatment (50 mg/mL, 100 mg/mL, and 200 mg/mL) significantly (p < 0.05) inhibited the
H2O2-induced (200 mM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 mg/mL and 100 mg/mL)
significantly (p < 0.05) ameliorated the H2O2-induced decrease in the expression level of antioxidant
enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4),
spermatogenesis-related protein such as inhibin-a, and specific sex hormone receptors (androgen receptor,
luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells.
Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related
nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST
(200 mg/kg) treatment. Taken together, GINST attenuated H2O2-induced oxidative stress in GC-2 cells
and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and
sex hormone receptors in aged rats.
Conclusion: GINST may be a potential natural agent for the protection against or treatment of oxidative
stress-induced male subfertility and aging-induced male subfertility.
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KEYWORD
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oxidative enzymes, Panax ginseng, pectinase, spermatogenesis, subfertility
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